Infección experimental cerebral con cisticercosis en ovejas

Katherine A. Sota; Javier A. Bustos; Manuela R. Verastegui; Luz Toribio; Nancy Chile; Noelia Angulo; Carla Cangalaya; Juan Calcina; Armando E. González; Robert H. Gilman; Héctor H. García

doi:10.17843/rpmesp.2022.393.11039

Authors

Katherine A. Sota Unidad de Cisticercosis, Instituto Nacional de Ciencias Neurológicas, Lima, Perú. https://orcid.org/0000-0002-8336-340X
Javier A. Bustos Unidad de Cisticercosis, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Centro de Salud Global, Universidad Peruana Cayetano Heredia, Lima, Perú. https://orcid.org/0000-0002-5797-3052
Manuela R. Verastegui Laboratorios de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Perú. https://orcid.org/0000-0002-7500-1353
Luz Toribio Centro de Salud Global, Universidad Peruana Cayetano Heredia, Lima, Perú. https://orcid.org/0000-0002-2818-4521
Nancy Chile Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, US. https://orcid.org/0000-0003-1851-8118
Noelia Angulo Laboratorios de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Perú. https://orcid.org/0000-0002-7555-5145
Carla Cangalaya Laboratorios de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Perú. https://orcid.org/0000-0002-3525-8445
Juan Calcina Escuela de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Perú. https://orcid.org/0000-0003-4464-3107
Armando E. González Escuela de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Perú. https://orcid.org/0000-0002-9198-6981
Robert H. Gilman Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, US. https://orcid.org/0000-0002-3704-9217
Héctor H. García Unidad de Cisticercosis, Instituto Nacional de Ciencias Neurológicas, Lima, Perú; Centro de Salud Global, Universidad Peruana Cayetano Heredia, Lima, Perú; Laboratorios de Investigación y Desarrollo, Universidad Peruana Cayetano Heredia, Lima, Perú. https://orcid.org/0000-0002-4633-6269

DOI:

https://doi.org/10.17843/rpmesp.2022.393.11039

Keywords:

Epilepsy, Neurocysticercosis, Sheep, Taenia solium, Cysticercosis

Abstract

Objective. To explore the feasibility of developing a sheep model of neurocysticercosis (NCC) by intracranial infection with T. solium oncospheres. Materials and methods. We carried out an experimental infection model of NCC in sheep. Approximately 10 T. solium oncospheres previously cultured for 30 days were inoculated intracranially into ten sheep. The oncospheres, in 0.1 mL of physiological saline, were injected into the parietal lobe through an 18-gauge needle. Results. After three months, granulomas were found in two sheep. In a third sheep we identified a 5 mm diameter cyst in the right lateral ventricle and histological evaluation confirmed that the cyst corresponded to a T. solium larva. Immunohistochemistry with monoclonal antibodies directed against membrane components and excretory/secretory antigens of the T. solium cyst was also used to confirm the etiology of the found granulomas. One of them showed reactivity to the monoclonal antibodies used, thus confirming that it was a cysticercus. Conclusion. This experiment is the proof of concept that it is possible to infect sheep with cysticercosis by intracranial inoculation.

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