Detection of Leishmania spp. based on the gene encoding HSP20

Authors

  • Ana M Montalvo Departamento de Parasitología, Instituto de Medicina Tropical Pedro Kourí. La Habana, Cuba.
  • Jorge Fraga Departamento de Parasitología, Instituto de Medicina Tropical Pedro Kourí. La Habana, Cuba.
  • Omaira Rodríguez Laboratorio de referencia e investigación en enfermedades tropicales de sanidad militar. Bogotá, Colombia.
  • Orestes Blanco Departamento de Parasitología, Instituto de Medicina Tropical Pedro Kourí. La Habana, Cuba.
  • Alejandro Llanos-Cuentas Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia. Lima, Perú.
  • Ana L. García Universidad de San Simón. Cochabamba, Bolivia.
  • Braulio M Valencia Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia. Lima, Perú.
  • Carlos Muskus Programa de Estudio y Control de Enfermedades Tropicales, Universidad de Antioquia. Medellín, Colombia.
  • Gert Van der Auwera Biomedical Sciences Department. Institute of Tropical Medicine of Antwerp. Amberes, Bélgica.
  • José M Requena Centro de Biología Molecular Severo Ochoa. Madrid, España.

DOI:

https://doi.org/10.17843/rpmesp.2014.314.112

Keywords:

Leishmania, Diagnosis, HSP20 heat-shock proteins

Abstract

Objectives. Explore a new target for molecular diagnosis of Leishmania. Materials and methods. We evaluated the utility of the gene that encodes the heat shock protein 20-kDa (Hsp20) for detecting Leishmania by polymerase chain reaction (PCR). PCR was normalized and analytical parameters were determined, as well as the validity and diagnostic accuracy, and concordance with the PCR - 18S. PCR-Hsp20 with DNA was obtained from a group of clinical samples from different sources. Results. The analytical parameters were adequate. The sensitivity obtained was 86% and the specificity was 100%. The concordance with the reference method was good (Ƙ = 0.731), which supports its potential use for diagnosis. The possibility of subsequent identification of the species by sequencing the amplified product gives an additional advantage. Conclusions. The usefulness of this gene as a new target for the detection of Leishmania was demonstrated. Because of its potential, it is recommended to improve the sensitivity of the method and to evaluate it in different endemic regions.

Downloads

Download data is not yet available.

Published

2014-12-01

Issue

Section

Research Articles

How to Cite

1.
Montalvo AM, Fraga J, Rodríguez O, Blanco O, Llanos-Cuentas A, García AL, et al. Detection of Leishmania spp. based on the gene encoding HSP20. Rev Peru Med Exp Salud Publica [Internet]. 2014 Dec. 1 [cited 2024 Apr. 14];31(4). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/112

Most read articles by the same author(s)