Concurrent infections by two dengue virus serotypes during and outbreak in northwestern Peru, 2008
DOI:
https://doi.org/10.17843/rpmesp.2010.271.1439Keywords:
Dengue, Dengue virus, Outbreak, Molecular biology, PeruAbstract
Objetives. To establish the existence of concurrent infections by different dengue virus (DENV) serotypes in an outbreak in the Northwestern in Peru during 2008. Material and methods. 73 serum samples from patients with dengue were analyzed during an outbreak that occurred in Northwestern in Peru between May and June 2008. Molecular biology techniques were used to serotype the DENV, thus, firstly the viral RNA viral was extracted using Viral QIAamp RNA mini kit (Qiagen, Valencia, California, USA), then the viral cDNA fragments were reverse transcripted and amplified by means of the Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) and the RT-Nested PCR region techniques and finally, genetic sequencing of the viral cDNA fragments were performed using the Big Dye Terminator v.3,1 kit. Results. The 73 dengue cases presented infections by different serotypes: 34 (46.6%) by DENV-3, 29 (39.7%) by DENV-1, 4 (5.5%) by DENV-4, and 6 (8.2%) concurrent infections by DENV-1 and DENV-3. The most frequent clinical manifestations observed among dengue patients were fever and headache (100%), myalgia (94.5%), ocular pain (83.6%), arthralgia (78.1%), shivers (63.0%), nausea/vomiting (38.4%), positive tourniquet test (30.1%), and rash (20.5%). All patients with concurrent infections presented light clinical course of dengue fever (Df) except one patient who had moderate hemorrhagic manifestations. Conclusion. This is the first Peruvian report of patients with concurrent infections of two DENV serotypes without severe clinical manifestations.Downloads
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Published
2010-03-31
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Section
Research Articles
How to Cite
1.
Mamani E, Figueroa D, García MP, Garaycochea M del C, Pozo EJ. Concurrent infections by two dengue virus serotypes during and outbreak in northwestern Peru, 2008. Rev Peru Med Exp Salud Publica [Internet]. 2010 Mar. 31 [cited 2024 Dec. 1];27(1). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/1439