Evaluación de una prueba de ELISA con antígeno metabólico de Fasciola hepatica para el diagnóstico de fasciolosis humana en Cajamarca, Perú.

Hernán Cornejo; Fátima Oblitas; Sandro Cruzado; William Quispe

doi:10.17843/rpmesp.2010.274.1529

Authors

Hernán Cornejo Laboratorio de Inmunoserologia, Laboratorio de Referencia Regional, Dirección Regional de Salud, Cajamarca, Perú. Biólogo Microbiólogo.
Fátima Oblitas Facultad de farmacia y Bioquímica, Universidad Privada Antonio Guillermo Urrelo, Cajamarca, Perú. Químico farmacéutico.
Sandro Cruzado Facultad de farmacia y Bioquímica, Universidad Privada Antonio Guillermo Urrelo, Cajamarca, Perú. Químico farmacéutico.
William Quispe Laboratorio de Zoonosis Parasitaria, Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú. Biólogo Microbiólogo.

DOI:

https://doi.org/10.17843/rpmesp.2010.274.1529

Keywords:

Fascioliasis/diagnosis, Fasciola hepatica, Immunologic test, Sensitivity and specificity, Peru

Abstract

Metabolic (excretion/secretion) antigen was obtained from sheep infected with Fasciola hepatica, with a 1005 µg/µL of protein concentration, composed principally by proteins of molecular weight between 1.2 and 170 KDa. Bands of 170, 150, 31, 24, 18-14 and 10 kDa were detected. With this antigen an ELISA test was developed and the cut off was determined in 0.140. We evaluated 33 serums of patient with fascioliasis confirmed by visualization of eggs in feces, 177 serums of persons without fascioliasis from endemic rural areas of Cajamarca and 88 serums of patients with others parasitic and bacterial infections. We found a 97.0% of sensitivity, 96.6 specificity, 78.1% predictive positive value, 99.6 % predictive negative value. In 9/88 serums was found cross reactions. We recommended the implementation and use of this test for the fascioliasis diagnosis.