Purification of antigenic fraction 27-28 kDa from the metabolic antigen from metabolic secreted-excreted from Fasciola hepatica

Authors

  • Isidro Antitupa Laboratorio de Zoonosis Parasitaria, Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • William Quispe Laboratorio de Zoonosis Parasitaria, Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • Jhon Mayo Laboratorio de Zoonosis Parasitaria, Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • Fanny Valverde Laboratorio de Zoonosis Parasitaria, Instituto Nacional de Salud. Lima, Perú. Técnico de Laboratorio
  • Elizabeth Sanchez Laboratorio de Zoonosis Parasitaria, Instituto Nacional de Salud. Lima, Perú. Biólogo. Magíster en Biología Parasitaria. Doctor en Ciencias.

DOI:

https://doi.org/10.17843/rpmesp.2014.312.48

Keywords:

Fasciola hepatica, Purification, Chromatography

Abstract

Antigenic fractions of 27-28 kDa from Fasciola hepatica were purified by size-exclusion chromatography for use in the diagnosis of human fasciolosis. Excretion and secretion antigens were obtained from living adult flukes collected from sheep and cattle liver, and cultured in minimum essential medium. The reactivity of the purified antigen and efficacy were assessed by immunoblot test using four sera with human fascioliasis; four sera with other parasites, and two negative sera. We conclude that the purified antigenic fractions do not cross-react with other parasites by immunoblot. Therefore, purified proteins are considered as potential candidates to be used for the diagnosis of human fascioliasis.

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Published

2014-07-02

Issue

Section

Research Articles

How to Cite

1.
Antitupa I, Quispe W, Mayo J, Valverde F, Sanchez E. Purification of antigenic fraction 27-28 kDa from the metabolic antigen from metabolic secreted-excreted from Fasciola hepatica. Rev Peru Med Exp Salud Publica [Internet]. 2014 Jul. 2 [cited 2024 Jun. 15];31(2). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/48

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