Purification of antigenic fraction 27-28 kDa from the metabolic antigen from metabolic secreted-excreted from Fasciola hepatica
Keywords:Fasciola hepatica, Purification, Chromatography
AbstractAntigenic fractions of 27-28 kDa from Fasciola hepatica were purified by size-exclusion chromatography for use in the diagnosis of human fasciolosis. Excretion and secretion antigens were obtained from living adult flukes collected from sheep and cattle liver, and cultured in minimum essential medium. The reactivity of the purified antigen and efficacy were assessed by immunoblot test using four sera with human fascioliasis; four sera with other parasites, and two negative sera. We conclude that the purified antigenic fractions do not cross-react with other parasites by immunoblot. Therefore, purified proteins are considered as potential candidates to be used for the diagnosis of human fascioliasis.
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How to Cite
Antitupa I, Quispe W, Mayo J, Valverde F, Sanchez E. Purification of antigenic fraction 27-28 kDa from the metabolic antigen from metabolic secreted-excreted from Fasciola hepatica. Rev Peru Med Exp Salud Publica [nternet]. 2014 Jul. 2 [cited 2023 Jun. 1];31(2). vailable from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/48
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