Molecular identification of Aspergillus fumigatus isolated from patients with invasive aspergillosis
DOI:
https://doi.org/10.17843/rpmesp.2019.361.3765Keywords:
Aspergillus fumigatus, Invasive Pulmonary Aspergillosis, Molecular typing, PeruAbstract
The objective of the study was to identify molecularly-isolated strains of Aspergillus from patients with invasive aspergillosis (IA); these strains were primarily typed as Aspergillus fumigatus sensu lato by conventional phenotypic methods. We worked with 20 strains from the mycology section of the Institute of Tropical Medicine "Daniel A. Carrión." To obtain the fungal DNA, thermal shock, enzymatic treatment, and silica gel column techniques were used; and it was stored at -20°C to preserve it. The real-time polymerase chain reaction (qPCR) procedure included fluorochrome-labeled primers, which amplified the specific sequences of A. fumigatus. Fluorescence was measured with the thermocycler at the end of the hybridization phase of each cycle. It was molecularly-identified that only 50% of the strains studied belong to the species Aspergillus fumigatus sensu stricto.Downloads
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Published
2019-03-22
Issue
Section
Brief Report
How to Cite
1.
Miranda J, Pinto J, Faustino M, Sánchez-Jacinto B, Ramirez F. Molecular identification of Aspergillus fumigatus isolated from patients with invasive aspergillosis. Rev Peru Med Exp Salud Publica [Internet]. 2019 Mar. 22 [cited 2024 Dec. 30];36(1):87-92. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/3765