Molecular identification of Aspergillus fumigatus isolated from patients with invasive aspergillosis

Authors

  • Jacqueline Miranda Clínica Centenario Peruano Japonesa. Lima, Perú. Tecnólogo médico
  • Joseph Pinto Universidad Privada San Juan Bautista. Lima, Perú. biólogo
  • Margot Faustino Universidad Peruana Cayetano Heredia. Lima, Perú. Tecnólogo médico
  • Billy Sánchez-Jacinto Universidad Peruana Cayetano Heredia. Lima, Perú. Tecnólogo médico
  • Fabrizio Ramirez Universidad Privada San Juan Bautista. Lima, Perú. médico cirujano

DOI:

https://doi.org/10.17843/rpmesp.2019.361.3765

Keywords:

Aspergillus fumigatus, Invasive Pulmonary Aspergillosis, Molecular typing, Peru

Abstract

The objective of the study was to identify molecularly-isolated strains of Aspergillus from patients with invasive aspergillosis (IA); these strains were primarily typed as Aspergillus fumigatus sensu lato by conventional phenotypic methods. We worked with 20 strains from the mycology section of the Institute of Tropical Medicine "Daniel A. Carrión." To obtain the fungal DNA, thermal shock, enzymatic treatment, and silica gel column techniques were used; and it was stored at -20°C to preserve it. The real-time polymerase chain reaction (qPCR) procedure included fluorochrome-labeled primers, which amplified the specific sequences of A. fumigatus. Fluorescence was measured with the thermocycler at the end of the hybridization phase of each cycle. It was molecularly-identified that only 50% of the strains studied belong to the species Aspergillus fumigatus sensu stricto.

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Author Biography

  • Margot Faustino, Universidad Peruana Cayetano Heredia. Lima, Perú. Tecnólogo médico

     

     

Published

2019-03-22

Issue

Section

Brief Report

How to Cite

1.
Miranda J, Pinto J, Faustino M, Sánchez-Jacinto B, Ramirez F. Molecular identification of Aspergillus fumigatus isolated from patients with invasive aspergillosis. Rev Peru Med Exp Salud Publica [Internet]. 2019 Mar. 22 [cited 2024 Apr. 21];36(1):87-92. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/3765

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