Evaluación de las pruebas DOT BLOT y aglutinación de látex para el diagnóstico de cisticercosis en Perú

Eduardo Miranda-Ulloa; Roxana Sandoval-Ahumada; Eduardo Ayala; Juan Vásquez-Ampuero

doi:10.17843/rpmesp.2014.312.50

Authors

Eduardo Miranda-Ulloa Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo, magíster en Microbiología.
Roxana Sandoval-Ahumada Hospital Nacional Hipólito Unanue. Lima, Perú. Médico patólogo.
Eduardo Ayala Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo.
Juan Vásquez-Ampuero Facultad de Medicina Veterinaria, Universidad Nacional Hermilio Valdizán. Huánuco, Perú. Médico veterinario.

DOI:

https://doi.org/10.17843/rpmesp.2014.312.50

Keywords:

Cysticercosis, Enzyme-linked immunosorbent assay, Latex fixation tests, Sensitivity and specificity

Abstract

In order to evaluate dot blot tests and latex agglutination for the detection of human cysticercosis with liquid antigen of Taenia solium cysticerci, 125 human sera were used, of which 60 were from people with cysticercosis confirmed by Western Blot, 45 with other parasitic diseases and 20 apparently healthy. The optimal concentration of antigen to impregnate dot blot strips was 0.01 ug/uL, and to impregnate the latex particles was 0.092 ug/uL. For the dot blot test, a sensitivity of 100% and specificity of 87.7% was found. For latex agglutination, a sensitivity of 93.3% and specificity of 89.2% was found. Both tests may be useful and feasible to implement alternatives of serological diagnosis in laboratories in endemic areas of Peru.