Cytotoxic activity of the chloroform fraction of Piper aduncum and its effect on the cell cycle in gastric cancer cell lines

Authors

  • Ana Mayanga-Herrera Laboratorio de Cultivo Celular e Inmunología, Universidad Científica del Sur, Lima, Perú. Instituto de Medicina Regenerativa, Universidad Científica del Sur, Lima, Perú. Bióloga genetista biotecnóloga, maestra en Ciencias con especialidad en Biotecnología https://orcid.org/0000-0002-4633-9857
  • Salyoc Tapia-Rojas Laboratorio de Cultivo Celular e Inmunología, Universidad Científica del Sur, Lima, Perú. Instituto de Medicina Regenerativa, Universidad Científica del Sur, Lima, Perú. bachiller en Genética y Biotecnología https://orcid.org/0000-0002-7735-3336
  • Alejandro Fukusak- Yoshizawa Instituto de Medicina Regenerativa, Universidad Científica del Sur, Lima, Perú. Laboratorio de Química y Bioquímica de los Productos Naturales, Universidad Científica del Sur, Lima, Perú. químico, magister scientiae https://orcid.org/0000-0001-5479-8415
  • Álvaro Marcelo-Rodríguez Instituto de Medicina Regenerativa, Universidad Científica del Sur, Lima, Perú. Laboratorio de Química y Bioquímica de los Productos Naturales, Universidad Científica del Sur, Lima, Perú. biólogo, doctor en Bioquímica https://orcid.org/0000-0003-2031-6806
  • José Amiel-Pérez Instituto de Medicina Regenerativa, Universidad Científica del Sur, Lima, Perú. químico farmacéutico, doctor en Farmacia y Bioquímica https://orcid.org/0000-0003-4455-3195

DOI:

https://doi.org/10.17843/rpmesp.2020.373.5157

Keywords:

Gastric Cancer, Cytotoxicity, Cell Cycle, Chloroform, Metastasis

Abstract

Objectives: To evaluate the cytotoxic activity of the chloroform fraction of the Piper aduncum methanolic extract (PAMoCl) and its effect on the cell cycle in two gastric cancer cell lines: AGS and KATO III. Materials and methods: The cytotoxic effect of PAMoCl was evaluated in cell lines AGS and KATO III. The following PAMoCl concentrations were tested, 1.25, 2.5, 5, 10, 20, 40, 80 and 160 μg/mL. Resazurine was used to evaluate cell viability. In the cell cycle assay, the cells were treated with 19.62 μg/mL and 39.23 μg/mL of PAMoCl for AGS as well as 87.49 μg/mL and 160 μg/mL for KATO III. After 24 hours both cell lines were analyzed by flow cytometry. Results: PAMoCl showed cytotoxic activity, inhibiting cell growth by 50%. It presented a (IC50) of 39.23 μg/mL and 87.49 μg/mL at 24 hours and a (IC50) of 49.47 μg/mL and 64.68 μg/mL at 48 hours against AGS and KATO III cell lines, respectively. In addition, it was observed that PAMoCl has an effect on the cell cycle, it causes an accumulation of cells in the G2/M phase. Conclusions: PAMoCl contains secondary metabolites with cytotoxic activity that have an effect on the G2/M phase of the cell cycle, in two gastric cancer cell lines, both primary and metastatic. The results of this study will allow us to deepen the search for more effective active ingredients found in PAMoCl for eliminating gastric cancer cells, but with less toxicity for healthy cells.

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Published

2020-07-06

Issue

Section

Original Article

How to Cite

1.
Mayanga-Herrera A, Tapia-Rojas S, Fukusak- Yoshizawa A, Marcelo-Rodríguez Álvaro, Amiel-Pérez J. Cytotoxic activity of the chloroform fraction of Piper aduncum and its effect on the cell cycle in gastric cancer cell lines. Rev Peru Med Exp Salud Publica [Internet]. 2020 Jul. 6 [cited 2024 Oct. 13];37(3):471-7. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/5157