Procedure for culture and identification of stem cells from human lipoaspirate for research purposes
DOI:
https://doi.org/10.17843/rpmesp.2020.373.5201Keywords:
Stem Cells, Regenerative Medicine, Adipose Tissue, Cell Separation, Primary Cell CultureAbstract
Human stem cells are born with the creation of life itself and some of them remain throughout life. Therefore, they can be found in adult tissues and used for basic and applied research. Currently, in our country there is a growing interest in the study and application of stem cells; however, little is known about the identification procedure. For this reason, this study aims to present, from a practical point of view, a procedure for the culture and identification of stem/stromal cells obtained from human lipoaspirate (Adipose Stem Cells), for research purposes. This procedure includes the immunophenotype characterization, cell differentiation potential, gene expression and cell culture quality control; and will serve as support for Peruvian scientific community professionals who wish to develop this line of research.Downloads
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Published
2020-08-12
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Special Section
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Copyright (c) 2020 Revista Peruana de Medicina Experimental y Salud Pública
This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
1.
Tapia-Rojas S, Mayanga-Herrera A, Enciso-Gutiérrez J, Centurion P, Amiel-Pérez J. Procedure for culture and identification of stem cells from human lipoaspirate for research purposes. Rev Peru Med Exp Salud Publica [Internet]. 2020 Aug. 12 [cited 2024 Nov. 13];37(3):547-53. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/5201