Effect of polivalent bothropic antivenom on phospholipase A2, L-Amino acid oxidase and hyaluronidase from peruvian snake venom

Authors

  • Julio Cesar Mendoza Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Biólogo.
  • Fanny Lazo Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Biólogo.
  • Liliana Yarlequé Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Obstetriz.
  • Nora Cecilia Ruiz Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Biólogo.
  • Armando Yarlequé Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Biólogo.
  • Silvia Pessah Centro Nacional de Productos Biológicos, Instituto Nacional de Salud. Lima, Perú. Médico.
  • Vicky Flores Centro Nacional de Productos Biológicos, Instituto Nacional de Salud. Lima, Perú. Químico farmaceútico.
  • César Bonilla Centro Nacional de Productos Biológicos, Instituto Nacional de Salud. Lima, Perú. Biólogo.

DOI:

https://doi.org/10.17843/rpmesp.2008.252.1254

Keywords:

Snake venoms, Hyaluronoglucosaminidase, Phospholidases A2, Hialuronidase, L-amino acid oxidase, Antivenins

Abstract

Bothrops sp. snakes causing the largest number of cases of ophidism in Peru, their venom contain several enzymes related to poison spreading, miotoxic and platelet aggregation disturbances. Objectives. The inhibiting capacity of liquid polivalent bothropic antivenom from Instituto Nacional de Salud (INS) has been evaluated on phospholipase A2 (PLA2), L amino acid oxidase (LAO) and hyaluronidase activities using B. atrox, B. barnetti, B. brazili and B. pictus venoms. Material and methods. In each case on 45% egg yolk lipoprotein, Lleucina and O-dianisidine, as well as hyaluronic acid as substrates respectively, using for each enzyme ½, 1 and 2 doses of either natural and heated (37 °C during five days) antivenom, assayed in triplicate. Results. HA was more neutralized enzyme for antivenin, all venoms with the exception of B. brazili were totally inhibited at any dose. For LAO had values of inhibition of 68 to 100% using two doses of the antivenin, PLA2 was the least inhibited (70 to 80%) to two doses. With the heated antivenin was a decline of the inhibitory effect initially found. Conclusions. The measurement of the HA might serve as an indicator of the in vitro potency of antivenins, the bothropic antivenin produced by INS keeping in vitro conditions for inhibition of three major activities of the Peruvian snake venoms.

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Published

2008-06-30

Issue

Section

Research Articles

How to Cite

1.
Mendoza JC, Lazo F, Yarlequé L, Ruiz NC, Yarlequé A, Pessah S, et al. Effect of polivalent bothropic antivenom on phospholipase A2, L-Amino acid oxidase and hyaluronidase from peruvian snake venom. Rev Peru Med Exp Salud Publica [Internet]. 2008 Jun. 30 [cited 2024 Jun. 22];25(2). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/1254

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