Experimental efficacy of IgY antibodies produced in eggs against the venom of the peruvian snake Bothrops atrox

Authors

  • Julio C. Mendoza Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Biólogo.
  • Dan Vivas Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. Biólogo.
  • Edith Rodríguez Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. biólogo, magíster en Biología Molecular.
  • Rosio Inga Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. biólogo, magíster en Biología Molecular.
  • Gustavo Sandoval Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. biólogo, magíster en Biología Molecular.
  • Fanny Lazo Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. biólogo, magíster en Biotecnología.
  • Armando Yarlequé Laboratorio de Biología Molecular, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos. Lima, Perú. biólogo, doctor en Ciencias Biológicas.

DOI:

https://doi.org/10.17843/rpmesp.2012.291.310

Keywords:

Snake venoms, Antivenins, Neutralization, Bothrops atrox

Abstract

Objectives . To develop an immunization protocol in order to produce avian Ig y immunoglobulins against Bothrops atrox Peruvian snake venom and to evaluate its neutralizing capacity. Materials and methods . Six Hy Line Brown hens were immunized each two weeks using 500μg/doses of B. atrox venom in a period of two months. Each week, eggs were collected for Ig y isolation from yolk using two consecutive steps with caprilic acid and ammonium sulfate. Detection of Ig y anti- B. atrox were performed by double immunodiffusion, whereas title and cross-reactivity were analyzed using ELISA and Western Blot technics, respectively. f urthermore, letal dose (DL 50 ) and Medium Effective Dose (DE 50 ) were obtained by Probit analysis. Results . As a result of this protocol, chicken Ig y ’s were obtained in a concentration of 8,5 ± 1,35 mg/yolk mL. DE 50 from avian antivenom was 575 μL/venom mg. Cross-reactivity studies showed Bothrops atrox venom share more commom epitopes with Bothrops brazili (47%) than others Bothrops venoms showing Lachesis muta (19%) and Crotalus durissus (12%) venoms a low crossing reactivity, instead. Conclusions . Using this procedure, we could purify chicken Ig y with a neutralizant capacity of B. atrox venom which is comparable to the antivenom of equine origin and demonstrate its capacity as a immunoanalitical tool to evaluate the cross reactivity with others peruvian snakes.

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Published

2014-01-29

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Section

Research Articles

How to Cite

1.
Mendoza JC, Vivas D, Rodríguez E, Inga R, Sandoval G, Lazo F, et al. Experimental efficacy of IgY antibodies produced in eggs against the venom of the peruvian snake Bothrops atrox. Rev Peru Med Exp Salud Publica [Internet]. 2014 Jan. 29 [cited 2024 Nov. 2];29(1). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/310

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