Evaluation of two ELISA IgM assays in leptospirosis outbreak research

Authors

  • Manuel Céspedes Laboratorio de Leptospirosis, Centro Nacional en Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • Lourdes Balda Laboratorio de Leptospirosis, Centro Nacional en Salud Pública, Instituto Nacional de Salud. Lima, Perú. Técnica de laboratorio.
  • Martha Glenny Laboratorio de Leptospirosis, Centro Nacional en Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo.

DOI:

https://doi.org/10.17843/rpmesp.2008.253.1285

Keywords:

Leptospirosis, Disease outbreaks, Enzyme-linked immunosorbent assay, Diagnosis test

Abstract

Was assessed two rapid tests for serodiagnosis of acute leptospirosis, the ELISA IgM pool (Instituto Nacional de Salud, Lima), compared with a commercial ELISA IgM (PANBIO) against the gold standard microagglutination test (MAT). We examined 24 patients admitted to a hospital for investigation of a leptospirosis outbreak. It took two samples of serum, the first when they were hospitalized and the second when the consultation or discharged from the hospital. Leptospirosis were diagnosed in 19 of 24 patients by MAT. ELISA IgM pool was show a better sensitivity for acute samples that commercial IgM ELISA (73.7% vs 31.6%), positive (93.3 vs 85.7%) and negative (44.4 vs 23.5%) predictive values and same specificity (80%). In the evolution of convalescent sera had the same performance, sensitivity of 100% and specificity of 80%. The rapid test of ELISA IgM pool is highly sensitive and specific, Not requiring specialized equipment and are available for use in any laboratory.

Downloads

Download data is not yet available.

Downloads

Published

2008-09-30

Issue

Section

Research Articles

How to Cite

1.
Céspedes M, Balda L, Glenny M. Evaluation of two ELISA IgM assays in leptospirosis outbreak research. Rev Peru Med Exp Salud Publica [Internet]. 2008 Sep. 30 [cited 2024 Nov. 2];25(3). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/1285

Most read articles by the same author(s)