Evaluation of two ELISA IgM assays in leptospirosis outbreak research
DOI:
https://doi.org/10.17843/rpmesp.2008.253.1285Keywords:
Leptospirosis, Disease outbreaks, Enzyme-linked immunosorbent assay, Diagnosis testAbstract
Was assessed two rapid tests for serodiagnosis of acute leptospirosis, the ELISA IgM pool (Instituto Nacional de Salud, Lima), compared with a commercial ELISA IgM (PANBIO) against the gold standard microagglutination test (MAT). We examined 24 patients admitted to a hospital for investigation of a leptospirosis outbreak. It took two samples of serum, the first when they were hospitalized and the second when the consultation or discharged from the hospital. Leptospirosis were diagnosed in 19 of 24 patients by MAT. ELISA IgM pool was show a better sensitivity for acute samples that commercial IgM ELISA (73.7% vs 31.6%), positive (93.3 vs 85.7%) and negative (44.4 vs 23.5%) predictive values and same specificity (80%). In the evolution of convalescent sera had the same performance, sensitivity of 100% and specificity of 80%. The rapid test of ELISA IgM pool is highly sensitive and specific, Not requiring specialized equipment and are available for use in any laboratory.Downloads
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Published
2008-09-30
Issue
Section
Research Articles
How to Cite
1.
Céspedes M, Balda L, Glenny M. Evaluation of two ELISA IgM assays in leptospirosis outbreak research. Rev Peru Med Exp Salud Publica [Internet]. 2008 Sep. 30 [cited 2024 Nov. 21];25(3). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/1285