Rapid centrifugation assay standarization for dengue virus isolation

Authors

  • Miryam Palomino Escuela de Biología, facultad de Ciencias Naturales y Matemáticas, Universidad Nacional federico Villarreal. Lima, Perú. Biólogo.
  • Victoria Gutierrez Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo.
  • Ramses Salas Laboratorio de Biotecnología, facultad de Ciencias Naturales y Matemáticas, Universidad Nacional federico Villarreal. Lima, Perú. Biólogo.

DOI:

https://doi.org/10.17843/rpmesp.2010.271.1444

Keywords:

Dengue, Inmunologic Test, Viral isolation, Plate centrifugation

Abstract

The plate centrifugation assay was standardized for dengue virus isolation from serum samples. C6/36-HT cells were used determining the optimal values for centrifugation spin speed, inoculum, sera dilution, and incubation time. Then, 22 positive serum samples with viral isolation and viral strains of the four reference dengue virus serotypes were tested simultaneously by the standardized plate centrifugation method and the conventional tube culture. The isolations were typified by indirect immunofluorescent test using monoclonal antibodies. The plate centrifugation method was optimized to 200 µL of inoculum, dilution of sera 1/20, centrifugation speed at 1600 rpm/30 min, and sensitivity of 95,5% after 5 days post-inoculation. We concluded that the plate centrifugation method increased dengue virus isolation, with a significant reduction of the time of isolation for dengue virus.

Downloads

Download data is not yet available.

Downloads

Published

2010-03-31

Issue

Section

Research Articles

How to Cite

1.
Palomino M, Gutierrez V, Salas R. Rapid centrifugation assay standarization for dengue virus isolation. Rev Peru Med Exp Salud Publica [Internet]. 2010 Mar. 31 [cited 2024 Jun. 23];27(1). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/1444

Most read articles by the same author(s)