Neutralization of SARS-CoV-2 (lineage B.1.1) by hyperimmune llama (Lama glama) serum in Vero cell culture
DOI:
https://doi.org/10.17843/rpmesp.2023.403.12509Keywords:
Coronavirus, SARS-CoV-2, Lama glama, Neutralization Tests, COVID-19 SerotherapyAbstract
Objective. To evaluate the serological antibody response of a llama (Lama glama) to SARS-CoV-2 (B.1.1 lineage)
immunization and the neutralizing capacity of hyperimmune llama serum against SARS-CoV-2 virus
(B.1.1 lineage) in Vero cells. Materials and methods. A llama was immunized with inactivated SARS-CoV-2 (B.1.1 lineage). Serum samples were analyzed to evaluate the level of antibodies by ELISA, as well as reactivity to SARS-CoV-2 antigens by Western Blot. In addition, viral neutralization in cell cultures was assessed by the Plate Reduction Neutralization Test (PRNT). Results. Seroreactivity increased in the immunized llama from week 4 onwards. Antibody titers were the highest after the seventh immunization booster. Western blot results confirmed the positive ELISA findings, and immune serum antibodies recognized several viral proteins. The neutralization assay (PRNT) showed visible viral neutralization, which was in accordance with the ELISA and Western Blot results. Conclusions. The findings suggest that hyperimmune llama serum could constitute a source of therapeutic antibodies against SARS-CoV-2 infections (lineage B.1.1), and should be studied in
further research.
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Copyright (c) 2023 Verónica Yaniro, Silvia Capristano, Henri Bailon, Juan Lévano, Marco Galarza, David García, Omar Cáceres, Carlos Padilla, Harrison Montejo, Paquita García, Mary Celis, Silvia Seraylan, Yessica Garayar, Miryam Palomino
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