Detection of influenza A, B and subtypes A (H1N1) pdm09, A (H3N2) viruses by multiple QRT-PCR in clinical samples

Authors

  • Pool Marcos Laboratorio de Referencia Nacional de Virus Respiratorios, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Magister en Biología molecular
  • Maribel Huaringa Laboratorio de Referencia Nacional de Virus Respiratorios, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Tecnólogo médico
  • Nancy Rojas Laboratorio de Referencia Nacional de Virus Respiratorios, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo
  • Victoria Gutierrez Laboratorio de Referencia Nacional de Virus Respiratorios, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Biólogo
  • Sila Ruiton Laboratorio de Referencia Nacional de Virus Respiratorios, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Técnico
  • Emelda Gallardo Laboratorio de Referencia Nacional de Virus Respiratorios, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Técnico
  • Jorge Achata Laboratorio de Referencia Nacional de Virus Respiratorios, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. PhD en Biología molecular
  • Marco Galarza Laboratorio de Referencia Nacional de Biología Molecular y Biotecnología, Centro Nacional de Salud Pública, Instituto Nacional de Salud. Lima, Perú. Magister en Biología molecular

DOI:

https://doi.org/10.17843/rpmesp.2017.342.2054

Keywords:

Multiplex polymerase chain reaction, Influenza A virus, H1N1 subtype, H3N2 subtype

Abstract

Objectives. To describe the clinical and epidemiological characteristics of patients diagnosed with epidermolysis bullosa (EB) at the Instituto Nacional de Salud (INSN) in Lima, Peru; a National Reference Center for this disease. Material and methods. Observational, descriptive and transversal study. We reviewed the clinical histories and laboratory tests of patients diagnosed with EB treated in INSN from 1993 to 2015. Results. 93 patients were registered. The average age was 7.9 ± 5.6 years; 53.8% (n = 50) were boys. Clinical forms corresponded to dystrophic EB with 41 (44.1%) cases, simple EB with 39 (41.9%) union EB cases with 8 (8.6%) and Kindler syndrome with 4 (4.3%) cases. The clinical form could not be identified in a case. A total of 48 cases (51.6%) came from Lima and Callao, and 45 cases (48.4%) from other provinces of the country. Extracutaneous manifestations involved gastrointestinal (44.1%), ocular (37.6%), odontogenic (87.1%), and nutritional (79.6%) involvement, as well as pseudosindactilia (16.1%). Chronic malnutrition (71.6%), acute malnutrition (17.6%) and anemia (62.4%) were found. Mortality corresponded to 6 cases (6.5%). Conclusions. 93 cases of EB were reported in INSN, the predominant clinical presentation was the dystrophic form.

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Published

2017-06-28

Issue

Section

Original Article

How to Cite

1.
Marcos P, Huaringa M, Rojas N, Gutierrez V, Ruiton S, Gallardo E, et al. Detection of influenza A, B and subtypes A (H1N1) pdm09, A (H3N2) viruses by multiple QRT-PCR in clinical samples. Rev Peru Med Exp Salud Publica [Internet]. 2017 Jun. 28 [cited 2024 Apr. 24];34(2):192-200. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2054

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