Lymphocyte subsets, dendritic cells and cytokine profiles in mice with melanoma treated with Uncaria tomentosa

Authors

  • Iván Lozada-Requena Laboratorio de Inmunologia. Departamento de Ciencias Celulares y Moleculares. Facultad de Ciencias y Filosofia. Universidad Peruana Cayetano Heredia. Lima, Peru. EMINDES SAC (empresa de investigacion y desarrollo en cancer). Lima, Peru.
  • César Núñez Laboratorio de Inmunologia. Departamento de Ciencias Celulares y Moleculares. Facultad de Ciencias y Filosofia. Universidad Peruana Cayetano Heredia. Lima, Peru. EMINDES SAC (empresa de investigacion y desarrollo en cancer). Lima, Peru.
  • Yubell Alvárez Laboratorio de Inmunologia. Departamento de Ciencias Celulares y Moleculares. Facultad de Ciencias y Filosofia. Universidad Peruana Cayetano Heredia. Lima, Peru.
  • Laura Kahn Laboratorio de Inmunologia. Departamento de Ciencias Celulares y Moleculares. Facultad de Ciencias y Filosofia. Universidad Peruana Cayetano Heredia. Lima, Peru.
  • José Aguilar Laboratorio de Inmunologia. Departamento de Ciencias Celulares y Moleculares. Facultad de Ciencias y Filosofia. Universidad Peruana Cayetano Heredia. Lima, Peru.

DOI:

https://doi.org/10.17843/rpmesp.2015.324.1752

Keywords:

Uncaria tomentosa, Lymphocyte activation, Dendritic cells, Melanoma

Abstract

Objectives. To evaluate the immunomodulatory effect on lymphocyte subsets, dendritic cells (DC), Th1 / Th2 / Th17 and inflammatory cytokines on systemic level and/or in the tumor microenvironment of mice with or without melanoma. Materials and methods: Peripheral blood and/or primary tumors samples were obtained of mice with B16 melanoma treated or not with a hydroalcoholic extract of Uncaria tomentosa (UT) with 5.03% of pentacyclic oxindole alkaloids (UT-POA) obtained from the bark of the plant. All cell assays and cytokine measurements were performed by flow cytometry. Results. UT-POA systemically increased CD4/CD8a relation while cell activation was inversely proportional; increased the proportion of DCm; induced a pro-inflammatory Th1 profile and reduced Th17 response. TNF-α and IL-17A positively and negatively correlated with CD4/CD8a relation. Conclusions. The increase of Th1 (TNF-α) may result in the increase of CD4 or M1 macrophage activation. Although UT-POA shows increased DCm, is not dose-dependent. Th17(IL-17A) decreased can support the function of CD8a lymphocytes. UT-POA shows better systemic immunomodulatory effects than intratumoral.

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Published

2015-12-05

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Section

Original Article

How to Cite

1.
Lozada-Requena I, Núñez C, Alvárez Y, Kahn L, Aguilar J. Lymphocyte subsets, dendritic cells and cytokine profiles in mice with melanoma treated with Uncaria tomentosa. Rev Peru Med Exp Salud Publica [Internet]. 2015 Dec. 5 [cited 2024 Apr. 23];32(4):633-42. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/1752