TEM and CTX-M extended-spectrum beta-lactamase in Klebsiella spp and Escherichia coli isolates from inanimate surfaces of hospital environments

Authors

  • Marco Rivera-Jacinto Facultad de Ciencias de la Salud, Universidad Nacional de Cajamarca. Cajamarca, Peru.
  • Claudia Rodríguez-Ulloa Facultad de Ciencias de la Salud, Universidad Nacional de Cajamarca. Cajamarca, Peru.
  • René Flores Clavo Hospital Regional de Lambayeque. Lambayeque, Peru.
  • Luis Serquén López Hospital Regional de Lambayeque. Lambayeque, Peru.
  • Zhandra Arce Gil Universidad Catolica Santo Toribio de Mogrovejo. Lambayeque, Peru.

DOI:

https://doi.org/10.17843/rpmesp.2015.324.1768

Keywords:

beta-Lactamases, Cross infection, Klebsiella pneumoniae, Escherichia coli, Polymerase chain reaction

Abstract

The aim of the study was to determine the genotype of 15 ESBL strains of Enterobacteriaceae resistant to beta-lactams, isolated from inanimate surfaces and phenotypically characterized as producing extended-spectrum beta-lactamase. After evaluation and screening of the bacterial strains, a PCR was conducted to amplify fragments of 1078 bp and 544 bp corresponding to type TEM and CTX-M ESBL. Eleven strains presented both fragments at the time and only three had blaCTX-M. In conclusion, the presence of ESBL genes in cultures from the environment was demonstrated, some of which may belong to more than one type. This information could serve as a basis for implementing preventive measures to prevent the transmission of multiresistant bacteria from inanimate surfaces to patients, mainly in critical hospital areas.

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Published

2015-12-06

Issue

Section

Brief Report

How to Cite

1.
Rivera-Jacinto M, Rodríguez-Ulloa C, Flores Clavo R, Serquén López L, Arce Gil Z. TEM and CTX-M extended-spectrum beta-lactamase in Klebsiella spp and Escherichia coli isolates from inanimate surfaces of hospital environments. Rev Peru Med Exp Salud Publica [Internet]. 2015 Dec. 6 [cited 2024 Apr. 23];32(4):752-5. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/1768

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