Chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine

Authors

  • Karla Ríos-León Instituto de Ciencias Farmacéuticas y Recursos Naturales, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Químico farmacéutica
  • Cesar Fuertes-Ruiton Instituto de Ciencias Farmacéuticas y Recursos Naturales, Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos. Lima, Perú. Químico farmacéutico, magíster en Ciencias Farmacéuticas y Recursos Naturales
  • Jorge Arroyo Instituto de Investigaciones Clínicas, Facultad de Medicina, Universidad Nacional Mayor de San Marcos. Lima, Perú. Químico farmacéutico, doctor en Farmacia y Bioquímica
  • Julio Ruiz Instituto de Investigación en Química Biológica, Microbiología y Biotecnología “Marco Antonio Garrido malo”. Lima, Perú. químico farmacéutico, magíster en Microbiología.

DOI:

https://doi.org/10.17843/rpmesp.2017.341.2768

Keywords:

Colon cancer, Melocactus bellavistensis, alkaloid, 1.2-dimethylhydrazine, toxicity, chemopreventive effect

Abstract

Objectives. To determine the toxicity and chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine (DMH). Materials and methods. The alkaloid extract was obtained from the fleshy part of M. bellavistensis, and an acute toxicity test was then carried out on 30 mice of the Balb C57 strain. To assess its chemoprotective effect, colon cancer was induced in 45 Holtzman rats using DMH according to the following experimental design: one control group received 2 mL/kg sodium polysorbate, and four groups received 20 mg/kg DMH plus 0, 1, 5, or 10 mg/kg M. bellavistensis alkaloid extract. Results. With a sample of 5 g of alkaloid extract, an LD50 greater than 1000 mg/mL was determined in the acute toxicity test. Histological indicators revealed that the 5 and 10 mg/kg doses had significant anti-tumor activity with 100% neoplasia inhibition against DMHinduced colon cancer in rats. Conclusions. Under experimental conditions, the alkaloid extract of M. bellavistensis has a chemoprotective effect against DMH-induced colon cancer in rats.

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Published

2017-03-23

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Section

Original Article

How to Cite

1.
Ríos-León K, Fuertes-Ruiton C, Arroyo J, Ruiz J. Chemoprotective effect of the alkaloid extract of Melocactus bellavistensis against colon cancer induced in rats using 1,2-dimethylhydrazine. Rev Peru Med Exp Salud Publica [Internet]. 2017 Mar. 23 [cited 2024 May 25];34(1):70-5. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2768

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