Circulation of a different lineage of dengue virus serotype 2 American / Asian genotype in the peruvian Amazon, 2010

Authors

  • Enrique Mamani Laboratorio de Arbovirus – Instituto Nacional de Salud, Perú. Biólogo.
  • Carlos Álvarez Dirección Regional de Salud Loreto. Médico.
  • María García M Dirección Regional de Salud Loreto. Médico. Tecnólogo Médico.
  • Dana Figueroa Laboratorio de Arbovirus – Instituto Nacional de Salud, Perú. Biólogo.
  • Milady Gatti Dirección Regional de Salud Loreto. Biólogo.
  • Heinner Guio Laboratorio de Biología Molecular y Biotecnología – Instituto Nacional de Salud. Médico.
  • Susy Merino Laboratorio de Arbovirus – Instituto Nacional de Salud, Perú. Tecnólogo Médico.
  • Pedro Valencia Centro Nacional de Salud Pública - Instituto Nacional de Salud, Perú. Médico.
  • Carlos Calampa Dirección Regional de Salud Loreto. Médico.
  • Leticia Franco Laboratorio de Arbovirus y virus importados. Centro Nacional de Microbiología. Instituto de Salud Carlos III – Madrid-España. Bioquímica.

DOI:

https://doi.org/10.17843/rpmesp.2011.281.458

Keywords:

Dengue Virus Type 2, Genotype, Peru

Abstract

Our objective was to determine the genotype of the dengue virus type 2 (DENV-2) that circulated in the Amazon region of Peru between November 2010 and January 2011. We analyzed eight samples collected during dengue surveillance activities in the cities of Iquitos, Yurimaguas, Trujillo, Tarapoto and Lima between November 2010 and January 2011 that were sent to Insitituto Nacional de Salud. The viruses were isolated in C6/36 HT cell line. Viral RNA was extracted and the serotype (RT - PCR multiplex) and genotype (RT-Nested PCR of the region E/NS1) were determined. Finally, the E/ NS1 amplicons were sequenced and analyzed by phylogeny. The phylogenetic analysis revealed the introduction of a different lineage which entered in Peru by the end of 2010. These isolates found in Iquitos and other cities in Peru are closely related to DENV-2 isolates that circulated in Brazil during 2007 and 2008, associated with severe dengue cases and deaths. In conclusion, we detected the introduction of a different lineage of DENV-2 America / Asia genotype in Peru that could be associated with the presence of more severe cases.

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Published

2011-03-31

Issue

Section

Research Articles

How to Cite

1.
Mamani E, Álvarez C, García M M, Figueroa D, Gatti M, Guio H, et al. Circulation of a different lineage of dengue virus serotype 2 American / Asian genotype in the peruvian Amazon, 2010. Rev Peru Med Exp Salud Publica [Internet]. 2011 Mar. 31 [cited 2024 Jun. 23];28(1). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/458

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