Comparative genomic analysis of Peruvian strains of Mycobacterium tuberculosis

Authors

  • David Tarazona Laboratorio de Referencia Nacional de Biología Molecular y Biotecnología, Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú
  • Marco Galarza Laboratorio de Referencia Nacional de Biología Molecular y Biotecnología, Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú
  • Kelly S. Levano Laboratorio de Referencia Nacional de Biología Molecular y Biotecnología, Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú
  • Heinner Guio Laboratorio de Referencia Nacional de Biología Molecular y Biotecnología, Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú

DOI:

https://doi.org/10.17843/rpmesp.2016.332.2192

Keywords:

Tuberculosis, Genomic, Drug Resistant, Bacterial

Abstract

Objectives. To comparatively analyze three genomic sequences of Mycobacterium tuberculosis (MTB), including sensitive (INS-SEN), multi-drug-resistant (INS-MDR), and extremely drug-resistant (INS-XDR) strains, collected in Lima, Peru. Materials and Methods. Specific single nucleotide polymorphisms (SNPs) were identified in the INS SEN, INSMDR, and INS-XDR strains according to the inclusion/exclusion criteria. The three MTB genomes were compared and a molecular phylogeny was constructed with 27 MTB strains from other studies available from the Genbank database. Results. The specific SNPs in each genome were organized in clusters of orthologous groups (COGs). The genomic analysis allowed for the identification of a set of SNPs associated mainly with virulence determinants (family of mce proteins, polyketides, phiRv1, transposase, and methyltransferases, and other related to vitamin synthesis). A close correlation between the INS-MDR and INS-XDR strains was observed, with only a 6.1% difference in SNPs; however, the INS-SEN strain had 50.2% and 50.3% different SNPs from the MDR and XDR strains, respectively. The molecular phylogeny grouped the Peruvian strains within the LAM lineage and closely to the F11 and KZN strains from South Africa. Conclusions. High similarity (99.9%) was noted between the INS-SEN strain and the F11 South African strain with broad global scope, while the analysis of the INS-MDR and INS-XDR strains showed a likely expansion of the KZN family, a South African strain with high virulence and pathogenicity.

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Published

2016-05-31

Issue

Section

Original Article

How to Cite

1.
Tarazona D, Galarza M, Levano KS, Guio H. Comparative genomic analysis of Peruvian strains of Mycobacterium tuberculosis. Rev Peru Med Exp Salud Publica [Internet]. 2016 May 31 [cited 2024 May 30];33(2):256-63. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/2192

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