Nitrofuran resistance in Salmonella enterica from human consumption meat samples

Authors

DOI:

https://doi.org/10.17843/rpmesp.2020.371.4745

Keywords:

Drug resistance, Furazolidone, Salmonella

Abstract

The mechanisms of resistance to nitrofurans from 18 meat samples with Salmonella enterica (chicken: 15; beef: 2; pork: 1) collected in Lima (Peru) were analyzed. The isolates were serotyped and the susceptibility levels to furazolidone and nitrofurantoin [with and without the efflux pump inhibitor Phenyl-Arginine- β-naphthylamide (PAβN)], the presence of mutations in the snrA and cnr genes and the transferability of resistance by conjugation were established. Fifteen samples with S. infantis (13 from chicken samples), 2 with S. enteritidis and 1 with S. anatum were identified. All isolates except the S. anatum were resistant to both nitrofurans showing MICs (minimum inhibitory concentration) of furazolidone and nitrofurantoin of 32-64 μg/mL and 128-256 μg/mL, respectively. The addition of PAßN had no effect on the MIC levels. All nitrofuran-resistant isolates showed amino acid codon alterations at both snrA and cnr (S. infantis: snrA STOP-151; cnr STOP-137; S. enteritidis: snrA STOP-180; cnr STOP-179). No transferable mechanisms of nitrofuran resistance were detected.

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Published

2020-03-23

Issue

Section

Brief Report

How to Cite

1.
Martínez-Puchol S, Pons MJ, Ruiz-Roldán L, Laureano-Adame L, Corujo A, Ochoa TJ, et al. Nitrofuran resistance in Salmonella enterica from human consumption meat samples. Rev Peru Med Exp Salud Publica [Internet]. 2020 Mar. 23 [cited 2024 Jun. 18];37(1):99-103. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/4745

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