Diagnostic performance of the culture and susceptibility of Helicobacter pylori in peruvian patients: results from a sentinel laboratory

Authors

  • Maricruz Olano Laboratorio Mixto Internacional Andino Amazónico de Química de la Vida, Universidad Peruana Cayetano Heredia. Lima, Perú.Laboratorio Centinela de Helicobacter pylori, Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú.Licenciada en Biología https://orcid.org/0000-0003-1864-3963
  • Manuel Chu Laboratorio Mixto Internacional Andino Amazónico de Química de la Vida, Universidad Peruana Cayetano Heredia. Lima, Perú.Laboratorio Centinela de Helicobacter pylori, Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú.biólogo, maestro en Bioquímica y Biología Molecular https://orcid.org/0000-0001-8797-0293
  • Jesus Guzmán Laboratorio Mixto Internacional Andino Amazónico de Química de la Vida, Universidad Peruana Cayetano Heredia. Lima, Perú.Laboratorio Centinela de Helicobacter pylori, Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú.químico farmacéutico, maestro en Bioquímicay Biología Molecular https://orcid.org/0000-0002-4366-0258
  • Denis Castillo Laboratorio Mixto Internacional Andino Amazónico de Química de la Vida, Universidad Peruana Cayetano Heredia. Lima, Perú.Laboratorio Centinela de Helicobacter pylori, Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú.biólogo, maestro en Bioquímica y Biología Molecular https://orcid.org/0000-0003-1010-2353
  • Michel Sauvain Laboratorio Mixto Internacional Andino Amazónico de Química de la Vida, Universidad Peruana Cayetano Heredia. Lima, Perú.Laboratorio Centinela de Helicobacter pylori, Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Perú.químico farmacéutico, PhD en Farmacoquímica https://orcid.org/0000-0003-3919-532X

DOI:

https://doi.org/10.17843/rpmesp.2021.383.7256

Keywords:

Helicobacter pylori, Prevalence, Diagnosis, Drug Resistance, Drug Resistance Multiple

Abstract

Objective: To analyze the antimicrobial susceptibility of Helicobacter pylori to 5 reference antibiotics, in a population of 500 dyspeptic patients from the Gastroenterology Service of the Hospital Cayetano Heredia (n = 419) and the Clínica Cayetano Heredia (n = 81) in Lima, Peru. Materials and methods: Gastric biopsies were collected from 500 patients diagnosed with dyspepsia. From these biopsies, 273 H. pylori strains were isolated and cultured to confirm H. pylori infection by histological and culture diagnosis. Finally, antimicrobial susceptibility was analyzed using the broth microdilution method, and the resistance profiles of each antimicrobial and multi-resistance patterns were evaluated by statistical analysis. Results: The diagnosis of H. pylori infection by culture, compared to histological testing, reported a sensitivity of 83.8%, a specificity of 89.9% and an area under the curve (AUC) of 0.87 (95% CI: 0.84 to 0.90). The frequency of infection in the gastroenterology services of the Hospital Cayetano Heredia and Clinic was 56.6% (237/419) and 44.4% (36/81), respectively. An increase in antimicrobial resistance to Amoxicillin (45.1% / 29.6%), Levofloxacin (71.8%/ 74.1%) and Metronidazole (69.8% / 63.0%) was found in the Hospital and the Clinic, respectively. Multiple resistance patterns showed that the most frequent resistance (double and triple) was to Levofloxacin, Metronidazole and Amoxicillin. Conclusions: The antimicrobial resistance of H. pylori has increased compared to that reported in previous years. Furthermore, H. pylori multiple resistance presents high frequencies in infected patients. The broth microdilution method could be implemented in different hospitals in Peru as a surveillance tool for H. pylori antimicrobial resistance.

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Published

2021-09-30

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Original Article

How to Cite

1.
Olano M, Chu M, Guzmán J, Castillo D, Sauvain M. Diagnostic performance of the culture and susceptibility of Helicobacter pylori in peruvian patients: results from a sentinel laboratory. Rev Peru Med Exp Salud Publica [Internet]. 2021 Sep. 30 [cited 2024 May 23];38(3):406-11. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/7256