Resistome and comparative genomics of clinical isolates of diarrheagenic Escherichia coli from Lima, Peru

Authors

DOI:

https://doi.org/10.17843/rpmesp.2020.374.5240

Keywords:

Escherichia coli, Drug resistance, Phylogeny, Genomics

Abstract

In order to describe the genomic characteristics related to antimicrobial resistance and comparative genomics of diarrheagenic Escherichia coli (DEC), 14 DEC isolates from the strain collection of the Instituto Nacional de Salud (INS) were subjected to genome sequencing. We used bioinformatic procedures to analyze the obtained sequences in order to look for microbial resistance genes and genetic regions related to pathotypes and phylogroups. Several antimicrobial resistance determinants were detected, but the production of beta-lactamases and mutations associated to quinolone resistance were the most relevant. Additionally, we observed isolates of the same pathotype grouped in different phylogroups. The comparative genomics analysis showed a greater number of orthologous genes in isolates from the same pathotype and phylogroup. In conclusion, DEC isolates from Lima, Peru, showed resistance to multiple drugs; likewise, molecular and phylogenetic diversity was observed in several pathotypes and phylogroups.

Downloads

Download data is not yet available.

Author Biographies

  • Willi Quino, Instituto Nacional de Salud, Lima, Perú. Tecnólogo médico magíster en Microbiología
    Lima
  • Orson Mestanza, Instituto Nacional de Salud, Lima, Perú. biólogo/a magíster en Bioinformática
    Lima
  • Junior Caro-Castro, Instituto Nacional de Salud, Lima, Perú. biólogo/a bachiller en Microbiología y Parasitología
    Lima
  • Carmen Verónica Hurtado, Instituto Nacional de Salud, Lima, Perú. biólogo/a bachiller en Microbiología y Parasitología
    Lima
  • Ronnie G. Gavilán, Instituto Nacional de Salud, Lima, Perú. Escuela Profesional de Medicina Humana, Universidad Privada San Juan Bautista, Lima, Perú. biólogo/a doctor en Bioquímica y Biología Molecular
    Lima

Published

2020-11-18

Issue

Section

Brief Report

How to Cite

1.
Quino W, Mestanza O, Caro-Castro J, Hurtado CV, Gavilán RG. Resistome and comparative genomics of clinical isolates of diarrheagenic Escherichia coli from Lima, Peru. Rev Peru Med Exp Salud Publica [Internet]. 2020 Nov. 18 [cited 2024 Apr. 19];37(4):705-10. Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/5240

Most read articles by the same author(s)