Effect of bovine lactoferrin biofilms in the formation of clinical strains of enteroaggregative E. Coli

Authors

  • Cecilia Coveñas Facultad de Medicina, Universidad Peruana Cayetano Heredia. Lima, Perú. Médico cirujano
  • Georgette Deztre Facultad de Medicina, Universidad Peruana Cayetano Heredia. Lima, Perú. Médico cirujano
  • Diana Fernandez Facultad de Medicina, Universidad Peruana Cayetano Heredia. Lima, Perú. Médico cirujano
  • Susan Mosquito Facultad de Medicina, Universidad Peruana Cayetano Heredia. Lima, Perú. magíster en Microbiología
  • Theresa Ochoa Facultad de Medicina, Universidad Peruana Cayetano Heredia. Lima, Perú. School of Public Health, University of Texas. Texas, EE.UU. médico infectólogo pediatra

DOI:

https://doi.org/10.17843/rpmesp.2014.313.80

Keywords:

Lactoferrin, Enteroaggregative Escherichia coli, Biofilms

Abstract

Objectives. To determine the effect of bovine lactoferrin (BLF) in the formation of biofilms in clinical enteroaggregative Escherichia coli (EAEC) strains and whether this effect is independent of iron. Materials and methods. Two methods were used: (a) qualitative, by direct observation of optical microscopy, and (b) quantitative readings of the absorbance values using ELISA reader in the presence of bLf in concentrations of 0.01 mg mL and 1 mg/mL, with and without iron and no bLf (control). Analysis occurred in 122 strains of EAEC (60 strains from children with diarrhea and 62 healthy children) previously collected in a previous study of passive surveillance of diarrhea in the Southern Cone Lima. 31 strains of the same method were used for the qualitative study. Results. (A) Qualitative method: 31 strains were evaluated with and without iron. Without iron biofilm formation was 77% (24/31) in the control group versus 58% (14/31) with bLf of 0.01 mg/mL and 4% (1/31) with 1 mg/ml. Iron biofilm formation was 90% (28/31) in the control group versus 55% (17/31) with bLf of 0.01 mg/mL and 4% (1/31) with 1 mg/mL. (B) Quantitative method: without iron absorbance measured at OD 560 nm of the control group was 0.7 ± 0.5 versus 0.4 ± 0.3 with bLf 0.01 mg/mL and 0.3 ± 0.2 with bLf of 1 mg/mL (p<0.0001). This decrease in the presence of bLf included iron. Conclusions. bLf tends to decrease the formation of biofilms, showing an inhibitory effect in clinical isolates of EAEC; this effect is not iron-dependent.

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Published

2014-09-25

Issue

Section

Research Articles

How to Cite

1.
Coveñas C, Deztre G, Fernandez D, Mosquito S, Ochoa T. Effect of bovine lactoferrin biofilms in the formation of clinical strains of enteroaggregative E. Coli. Rev Peru Med Exp Salud Publica [Internet]. 2014 Sep. 25 [cited 2024 Nov. 8];31(3). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/80

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