Rendimiento diagnóstico de los antígenos de Leishmania braziliensis y Leishmania peruviana en el método de inmunoblot para la detección de la leishmaniasis tegumentaria americana

Nyshon Rojas-Palomino; Aidé Sandoval-Juarez; Gilmer Solis-Sánchez; Gloria Minaya-Gómez

doi:10.17843/rpmesp.2024.413.13231

Authors

Nyshon Rojas-Palomino Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú https://orcid.org/0000-0003-1303-3744
Aidé Sandoval-Juarez Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú https://orcid.org/0000-0001-5863-803X
Gilmer Solis-Sánchez Centro Nacional de Alimentación, Nutrición y Vida Saludable, Instituto Nacional de Salud, Lima, Perú. https://orcid.org/0000-0001-7084-088X
Gloria Minaya-Gómez Centro Nacional de Salud Pública, Instituto Nacional de Salud, Lima, Perú. https://orcid.org/0000-0002-0097-9568

DOI:

https://doi.org/10.17843/rpmesp.2024.413.13231

Keywords:

Leishmaniasis, Leishmania, Western Blotting, Leishmania braziliensis, Antigens, Sensitivity and Specificity

Abstract

This study aimed to determine the performance of Leishmania braziliensis and Leishmania peruviana antigens in the detection of ATL by using serum samples obtained between 2013 - 2016. The obtained soluble and excretion/secretion antigens were transferred to membrane nitrocellulose by immunoblot assay. The evaluation was carried out against sera confirmed for ATL, at a confidence level of 95%, determining that the soluble antigen of Leishmania braziliensis had a sensitivity of 87.7%, specificity of 100% and area under the curve of 0.95; on the other hand, Leishmania peruviana showed values of 92.3%, 95.7% and 0.94, respectively. According to the results, we recommend that the reported immunogenic regions should be characterized and analyzed in order to continue
with the development of recombinant and synthetic proteins, aimed at improving the efficiency of the serological diagnosis of the disease.

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