Molecular test genotype MTBDRPLUS, an alternative to rapid detection of multidrug resistance tuberculosis
DOI:
https://doi.org/10.17843/rpmesp.2012.291.314Keywords:
Tuberculosis, multidrug-resistant, Molecular diagnostic techniques, Mutation, Isoniazid, Rifampin, Validity of tests, PeruAbstract
The Genotype®MTBDRplus molecular test is a method that allows identification of the most frequent mutations associated with resistance to major first-line antituberculosis drugs, Isoniazid (INH) and Rifampicin (RfP). The aim of this study was to evaluate the performance of the molecular test with culture and smear- positive sputum samples. We evaluated 95 cultures and 100 sputum samples with resistance profiles previously determined by the reference method “Agar Plate Proportions” (APP). The molecular test from cultures showed a sensitivity of 100 %, 97,5 % and 96,97 % for RIf, INH and MDR respectively while from sputums the sensitivity was 95,65 %, 96,77 % and 95,24 % for RIf, INH and MDR respectively. We conclude that the molecular test Genotype®MTBDRplus is a very useful tool to detect resistance to isoniazid and rifampicin simultaneously (MDR-TB) in up to 72 hours from sputum samples or cultures.Downloads
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Published
2014-01-30
Issue
Section
Research Articles
How to Cite
1.
Asencios L, Galarza M, Quispe N, Vásquez L, Leo E, Valencia E, et al. Molecular test genotype MTBDRPLUS, an alternative to rapid detection of multidrug resistance tuberculosis. Rev Peru Med Exp Salud Publica [Internet]. 2014 Jan. 30 [cited 2024 Nov. 3];29(1). Available from: https://rpmesp.ins.gob.pe/index.php/rpmesp/article/view/314